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Stimuleret in situ reduktiv deklorering
Videnopsamling og screening af lokaliteter - Appendiksrapport
Jørgensen, T.H.; Scheutz, Charlotte; Durant, N.D.; Cox, E.; Bordum, N.E.; Rasmussen, P.; Bjerg, Poul Løgstrup
Publication date:
2005
Document Version
Også kaldet Forlagets PDF Link back to DTU Orbit
Citation (APA):
Jørgensen, T. H., Scheutz, C., Durant, N. D., Cox, E., Bordum, N. E., Rasmussen, P., & Bjerg, P. L. (2005).
Stimuleret in situ reduktiv deklorering: Videnopsamling og screening af lokaliteter - Appendiksrapport.
Miljøstyrelsen. Miljøprojekt Nr. 984
Stimuleret in situ reduktiv
deklorering. Vidensopsamling og screening af lokaliteter
Appendiksrapport
Torben Højbjerg Jørgensen, COWI A/S
Charlotte Scheutz, Danmarks Tekniske Universitet Neal D. Durant, GeoSyntec Consultants
Evan Cox, GeoSyntec Consultants Niels Erik Bordum, COWI A/S Poul Rasmussen, Fyns Amt
Poul Løgstrup Bjerg, Danmarks Tekniske Universitet
Miljøprojekt Nr. 984 2005
Teknologiudviklingsprogrammet for jord- og
Miljøstyrelsen vil, når lejligheden gives, offentliggøre rapporter og indlæg vedrørende forsknings- og udviklingsprojekter inden for miljøsektoren, finansieret af Miljøstyrelsens undersøgelsesbevilling.
Det skal bemærkes, at en sådan offentliggørelse ikke nødvendigvis betyder, at det pågældende indlæg giver udtryk for Miljøstyrelsens synspunkter.
Offentliggørelsen betyder imidlertid, at Miljøstyrelsen finder, at indholdet udgør et væsentligt indlæg i debatten omkring den danske miljøpolitik.
Indholdsfortegnelse
APPENDIKS A LITTERATUR DATABASE 4
APPENDIKS A1OVERSIGT OVER LITTERATUR VEDR.
LABORATORIEFORSØG 4
APPENDIKS A2OVERSIGT OVER LITTERATUR VEDR.
FELTUNDERSØGELSER 13
APPENDIKS B UDVALGTE CASE STUDIER MED
BIOAUGMENTATION 22
APPENDIKS C LOKALITETS- OG FELTUNDERSØGELSER 30
APPENDIKS D FELTMETODER 32
PUSH-PULL TESTS 32
IN SITU MIKROKOSMOS 32
PILOTFORSØG 33
APPENDIKS E RISIKOFORHOLD 35
FORHOLD I NORDAMERIKA 35
APPENDIKS F SCREENING AF LOKALITETER 39
APPENDIKS F.1NØRREGADE 4,ASSENS 39 APPENDIKS F.2 ADELGADE 138,BOGENSE 44 APPENDIKS F.3 DALUMVEJ 22-28,ODENSE 49 APPENDIKS F.4 DALUMVEJ 34B,ODENSE 54 APPENDIKS F.5 DALUMVEJ 62,ODENSE 59 APPENDIKS F.6 MIDDELFARTVEJ 126,ODENSE 64 APPENDIKS F.7 SANDERUMVEJ 113,ODENSE 69 APPENDIKS F.8 RØNNEVEJ 13-15NÆSBY 75 APPENDIKS F.9 RUGÅRDSVEJ 166,ODENSE 80 APPENDIKS F.10 RUGÅRDSVEJ 234–238,ODENSE 85 APPENDIKS F.11 SORTEBROVEJ 26,TOMMERUP 90 APPENDIKS F.12 BOGENSEVEJ 39,GRØNNEMOSE,ÅRUP 96 APPENDIKS F.13 INDUSTRIVIRKSOMHED,SVENDBORG 102
APPENDIKS G ERFARINGER FRA HOLLAND 108
...
Appendiks A Litteratur database
Appendiks A1 Oversigt over litteratur vedr. laboratorieforsøg Dette appendiks giver en skematisk oversigt over relevant litteratur om laboratorieundersøgelser. De væsentligste artikler er markeret med gult.
AuthorsYearGray or Scientific Literature ScaleSite Descrip- tionSite LocationGeologyConta- minantsInoculumApproach System DesignDechlorinationInitial conc. Electron donorTempe- rature C
Key Findings son,D.T., Mcdade,J.M., and Hughes,J.B. 2003. culation of DNAPL source zone to initiate ductive dechlorination of PCE, Environmental ience & Technology 37: 2525-2533.
2003SLab silty sandDechlorinating, methanogenic mixed culture enriched on PCE/lactate Sand box BatchPCE→VC/ethene 59.9uMlactate acetate HRC
12, 22, 35Elevated levels of TCE and cis-DCE were present in the source zone, but neither VC nor ethene were detected in the vicinity of NAPL. The results of this research indicated that adding dechlorinating cultures maybe useful in the application of source zone bioremediation but that dechlorination beyond cis-DCE may be limited to regions downgradient of the source zone. amson,D.T. and Parkin,G.F. 2001. Product stribution during transformation of multiple ntaminants by a high-rate, tetrachlorethene- chlorinating enrichment culture, Biodegradation : 337-348.
2001SLabAnaerobic digester sludge. Enrichment culture (on lactate) BatchPCE→VC/ethene/CO2 CT→DCM/CO2
200uM 6uM lactate (C-source: acetate)
The addition of both CT and PCE inhibited the transformation of the individual compounds and reduced the percentages recovered as (CO2)-C- 14. However, the magnitude of these reductions was not severe and appeared to be the result of slower overall transformation rather than a complete inhibition of mineralization pathways. son,D.T. and Parkin,G.F. 2000. Impact of ixtures of chlorinated aliphatic hydrocarbons on a gh-rate, tetraehloroethene-dechlorinating richment culture, Environmental Science & chnology 34: 1959-1965.
2000SLab Anaerobic digester sludge.Enrichment culture (on lactate) BatchPCE→ethene CT→DCM 1,1,-TCA→CA 200uM 20uM 20uM
lactate20A PCE-acclimated, lactate-enrichment culture that demonstrated the ability to rapidly transform PCE to ethene in conjunction with methanogenesis was able to degrade both CT and 1,1,1-TCA despite no previous exposure to these compounds. While the presence of <20 mu M of 1,1,1-TCA had little effect on PCE removal, the addition of 10-15 mu M of CT negatively impacted both the PCE and vinyl chloride (VC) transformation steps. gley,D.M., Lalonde,M., Kaseros,V., Stasiuk,K.E., d Sleep,B.E. 2000. Acclimation of anaerobic stems to biodegrade tetrachloroethene in the esence of carbon tetrachloride and chloroform, er Research 34: 171-178.
2000SLabAnaerobic digester sludge from a sewage treatment plant. Enrichment culture (on ethanol)
Batch ColumnPCE→cis-DCE CT→DCM 8uM etahnol yeast extract20The addition of 19 mu M carbon tetrachloride to microcosms completely inhibited PCE degradation, as did 4 mu M chloroform although 0.7 mu M chloroform was not inhibitory. llapragada,B.S., Stensel,H.D., Puhakka,J.A., and rguson,J.F. 1997. Effect of hydrogen on reductive chlorination of chlorinated ethenes, vironmental Science & Technology 31: 1728-1734.
1997SLab Enrichment culture (on lactate)Fluidized bed reactor Batch PCE→VC/ethene160uM lactate propinate H2 acetate
35The half-velocity coefficients for H-2 utilization by dechlorinators ranged from 12 to 28 ppm for the chloroethenes and are at least an order of magnitude lower than values reported for methanogens. This implies that dechlorinating bacteria can out-compete methanogens for H-2 utilization at low H-2 concentration. adley,P.M. and Chapelle,F.H. 1999. Methane as a oduct of chloroethene biodegradation under ethanogenic conditions, Environmental Science & chnology 33: 653-656.
1999SLabBed sediment (methanogenic and contaminated with low TCE, cis-DCE and VC conc.) Batch – sedimentTCE→CH4 VC→CH4
TCE: 550ug/L TCE: 370ug/LRadiometric detection headspace analyses of microcosms containing bed sediments from two geographically distinct sires indicated that 10-39% of the radiolabeled carbon transformed during anaerobic biodegradation of [1,2-C-14]trichloroethene (TCE) or [1,2-C-14]vinyl chloride (VC) under methanogenic conditions was ultimately incorporated into (CH4)-C-14. l,N., Jacob,F., Perrier,J., Fouillet,B., and ambon,P. 1998. Complete degradation of high ncentrations of tetrachloroethylene by a ethanogenic consortium in a fixed-bed reactor, urnal of Biotechnology 62: 133-141.
1998SLabDigested slugde from a waste water tyreatment plant. Methanogenic and sulfate- reducing mixed consortium
Fixed bed reactorPCE→ethene215uMmethanolThe dechlorination of PCE was efficient and complete for high concentrations: 40-215 mu M of PCE. Indeed, 215 mu M PCE was degraded rapidly at the rate 3 mu mol l(-1) h(-1) and with 98% PCE removal. No chlorinated products were accumulated in the effluent. The tracer experiments with C-13-labeled PCE indicated that the PCE was completely degraded to carbon in the biomass and CO2. r,C.S. and Hughes,J.B. 1998. Enrichment of high te PCE dechlorination and comparative study of ctate, methanol, and hydrogen as electron dopers stain activity, Environmental Science & chnology 32: 1817-1824.
1998SLabAnaerobic digester sludge from a sewage treatment plant. Enrichment culture (PCE/methanol). Aquifer- solids from a contaminated site. Enrichment culture (PCE/lactate/H2).
Batch ColumnPCE→VC/ethene6.7uMlactate methanol H2
25Columns were fed PCE (aqueous concentration of 5 mg/L) and either lactate, methanol, or H-2 as their electron donor. Comparisons between electron donors were formulated using the observed rates of PCE disappearance and the extent of dechlorination achieved in each column. Over a period exceeding 1 year, the observed PCE half-life in each column was found to decrease from approximately 13 h to slightly less than 2 h, regardless of electron donor fed.
